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Construction of a stable shuttle vector for high-frequency transformation in Pseudomonas syringae pv. syringae.

By P Mukhopadhyay, M Mukhopadhyay and D Mills

Abstract

A cryptic 80.3-kilobase plasmid, pOSU900, in Pseudomonas syringae pv. syringae strain J900 could be cured by treatment with mitomycin without affecting the pathogenicity of J900 on the host, Phaseolus vulgaris L. The replication region of pOSU900 was identified, subcloned, and modified for construction of a high-copy cloning vector. This vector could be transformed into Pseudomonas strains with high efficiency (ca. 10(6) transformants per microgram of DNA) and was very stable during growth of the host bacteria in planta

Topics: Research Article
Year: 1990
OAI identifier: oai:pubmedcentral.nih.gov:208456
Provided by: PubMed Central
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