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UV mutagenesis in Salmonella typhimurium is umuDC dependent despite the presence of samAB.

By W H Koch, T A Cebula, P L Foster and E Eisenstadt

Abstract

We investigated the role of the umuDC and samAB operons in the UV mutability of Salmonella typhimurium. umuDC is located on the chromosome, whereas samAB resides on the virulence plasmid pSLT. Using allele replacement and plasmid curing techniques, we found that UV mutability was eliminated when any of three different umuDC alleles (umuD1, umuC1, or umuD1 umuC1) were on the chromosome even when samAB was present. We conclude that samAB normally does not complement umuDC function in S. typhimurium

Topics: Research Article
Year: 1992
OAI identifier: oai:pubmedcentral.nih.gov:205931
Provided by: PubMed Central
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