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Overexpression of genes of an extreme thermophile Thermus thermophilus, in Escherichia coli cells.

By M Ishida and T Oshima


The 3-isopropylmalate dehydrogenase gene from an extreme thermophile, Thermus thermophilus, was not expressed in Escherichia coli unless a palindromic structure around the ribosome binding site was eliminated or a leader open reading frame was introduced into the upstream flanking region of the gene. This report suggests a way to increase the expression of this gene, with a high G+C content, in E. coli

Topics: Research Article
Year: 1994
OAI identifier: oai:pubmedcentral.nih.gov:205423
Provided by: PubMed Central
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