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Cloning and expression in Escherichia coli of the dnaK gene of Zymomonas mobilis.

By G P Michel


The DnaK protein of Zymomonas mobilis (DnaKz) was identified and found to be 80% identical to the DnaK protein of Escherichia coli on the basis of the sequence of the N-terminal 21 amino acids. The dnaKz gene was cloned and found to be expressed in a thermosensitive dnaK mutant of Escherichia coli. Expression of the foreign gene restored a thermoresistant phenotype but failed to modulate the heat shock response in E. coli

Topics: Research Article
Year: 1993
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Provided by: PubMed Central
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