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Purification of the LysR family regulator, ClcR, and its interaction with the Pseudomonas putida clcABD chlorocatechol operon promoter.

By W M Coco, M R Parsek and A M Chakrabarty

Abstract

Previous studies have shown that the clcABD operon is under the transcriptional control of the LysR-type activator ClcR. In this study, the conditions leading to its aggregation were avoided and ClcR was purified and confirmed by amino-terminal sequencing. Gel filtration indicated that ClcR exists as a dimer in solution. The DNase I footprint of ClcR was determined. The binding properties of ClcR and the catechol operon regulator, CatR, were compared

Topics: Research Article
Year: 1994
DOI identifier: 10.1128/jb.176.17.5530-5533.1994
OAI identifier: oai:pubmedcentral.nih.gov:196742
Provided by: PubMed Central
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