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siRNAs can function as miRNAs

By John G. Doench, Christian P. Petersen and Phillip A. Sharp

Abstract

With the discovery of RNA interference (RNAi) and related phenomena, new regulatory roles attributed to RNA continue to emerge. Here we show, in mammalian tissue culture, that a short interfering RNA (siRNA) can repress expression of a target mRNA with partially complementary binding sites in its 3′ UTR, much like the demonstrated function of endogenously encoded microRNAs (miRNAs). The mechanism for this repression is cooperative, distinct from the catalytic mechanism of mRNA cleavage by siRNAs. The use of siRNAs to study translational repression holds promise for dissecting the sequence and structural determinants and general mechanism of gene repression by miRNAs

Topics: Research Communication
Publisher: Cold Spring Harbor Laboratory Press
Year: 2003
DOI identifier: 10.1101/gad.1064703
OAI identifier: oai:pubmedcentral.nih.gov:195999
Provided by: PubMed Central
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