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Minimal Requirements for Exponential Growth of Lactococcus lactis

By Peter Ruhdal Jensen and Karin Hammer


A minimal growth medium containing glucose, acetate, vitamins, and eight amino acids allowed for growth of Lactococcus lactis subsp. lactis, with a specific growth rate in batch culture of μ = 0.3 h(-1). With 19 amino acids added, the growth rate increased to μ = 0.7 h(-1) and the exponential growth phase proceeded until high cell concentrations were reached. We show that morpholinepropanesulfonic acid (MOPS) is a suitable buffer for L. lactis and may be applied in high concentrations

Topics: Physiology and Biotechnology
Year: 1993
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Provided by: PubMed Central
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