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Molecular and microscopic identification of sulfate-reducing bacteria in multispecies biofilms.

By R I Amann, J Stromley, R Devereux, R Key and D A Stahl

Abstract

The population architecture of sulfidogenic biofilms established in anaerobic fixed-bed bioreactors was characterized by selective polymerase chain reaction amplification and fluorescence microscopy. A region of the 16S rRNA common to resident sulfate-reducing bacteria was selectively amplified by the polymerase chain reaction. Sequences of amplification products, with reference to a collection of 16S rRNA sequences representing most characterized sulfate-reducing bacteria, were used to design both general and specific hybridization probes. Fluorescent versions of these probes were used in combination with fluorescence microscopy to visualize specific sulfate-reducing bacterial populations within developing and established biofilms

Topics: Research Article
Year: 1992
OAI identifier: oai:pubmedcentral.nih.gov:195292
Provided by: PubMed Central
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