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Comparison of Purified Alpha-Toxins from Various Strains of Staphylococcus aureus

By Ronald L. Goode and Jack N. Baldwin

Abstract

Alpha-toxin from five strains of Staphylococcus aureus, including Wood 46, was purified by isoelectric focusing. The alpha-toxins obtained from different strains were similar. The isoelectric point of the purified toxins was 8.65 ± 0.15. Sharp concentration peaks were not always obtained. In the ultracentrifuge the alpha-toxins migrated usually as three peaks which could be dissociated with propionic acid to yield one peak. A single line of identity was obtained in immunoelectrophoresis when a heterologous antiserum was reacted with the five purified toxins. It was concluded that the widespread use of the Wood 46 strain for the production of alpha-toxin is justified

Topics: Clinical Microbiology and Immunology
Year: 1974
OAI identifier: oai:pubmedcentral.nih.gov:186598
Provided by: PubMed Central
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