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DNA Probe for Lactobacillus delbrueckii

By Michèle Delley, Beat Mollet and Herbert Hottinger

Abstract

From a genomic DNA library of Lactobacillus delbrueckii subsp. bulgaricus, a clone was isolated which complements a leucine auxotrophy of an Escherichia coli strain (GE891). Subsequent analysis of the clone indicated that it could serve as a specific DNA probe. Dot-blot hybridizations with over 40 different Lactobacillus strains showed that this clone specifically recognizes L. delbrueckii subsp. delbrueckii, bulgaricus, and lactis. The sensitivity of the method was tested by using an α-32P-labeled DNA probe

Topics: Genetics and Molecular Biology
Year: 1990
OAI identifier: oai:pubmedcentral.nih.gov:184540
Provided by: PubMed Central
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