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Construction of a Biotin-Overproducing Strain of Serratia marcescens

By Naoki Sakurai, Yuji Imai, Makoto Masuda, Saburo Komatsubara and Tetsuya Tosa

Abstract

We have isolated mutants resistant to acidomycin, a biotin analog, from Serratia marcescens Sr41. Strain SB304, resistant to 0.5 mg of acidomycin (frequently called actithiazic acid) per ml, produced 5 mg of d-biotin per liter of a medium containing sucrose and urea. Strain SB412, which was isolated from SB304 on a minimal agar plate containing 2 mg of acidomycin per ml and 0.1 mg of 5-(2-thienyl)-valeric acid per ml, produced 20 mg of d-biotin per ml. The two enzymes related to biotin synthesis were found to be released from biotin-mediated feedback repression in these mutants. Transductional analysis revealed that SB412 had acquired at least two mutations, one in the biotin operon locus and the other in an unknown locus distant from the biotin operon locus

Topics: Physiology and Biotechnology
Year: 1993
OAI identifier: oai:pubmedcentral.nih.gov:182377
Provided by: PubMed Central
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