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Mutations affecting substrate specificity of the Bacillus subtilis multidrug transporter Bmr.

By K A Klyachko, S Schuldiner and A A Neyfakh

Abstract

The Bacillus subtilis multidrug transporter Bmr, a member of the major facilitator superfamily of transporters, causes the efflux of a number of structurally unrelated toxic compounds from cells. We have shown previously that the activity of Bmr can be inhibited by the plant alkaloid reserpine. Here we demonstrate that various substitutions of residues Phe143 and Phe306 of Bmr not only reduce its sensitivity to reserpine inhibition but also significantly change its substrate specificity. Cross-resistance profiles of bacteria expressing mutant forms of the transporter differ from each other and from the cross-resistance profile of cells expressing wild-type Bmr. This result strongly suggests that Bmr interacts with its transported drugs directly, with residues Phe143 and Phe306 likely to be involved in substrate recognition

Topics: Research Article
Year: 1997
DOI identifier: 10.1128/jb.179.7.2189-2193.1997
OAI identifier: oai:pubmedcentral.nih.gov:178954
Provided by: PubMed Central
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