Isolation and characterization of a mutation that alters the substrate specificity of the Escherichia coli glucose permease.
We isolated 10 mannitol-positive mutants from a mannitol-negative Escherichia coli strain. These mutations mapped within ptsG, encoding the glucose permease (EIIGlc), and resulted in a G-320-to-V substitution that allows EIIGlc to transport mannitol. Gly-320 lies within a putative transmembrane helix of EIIGlc that may be involved in substrate recognition
DOI identifier: 10.1128/jb.178.3.940-942.1996
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