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Molecular cloning with a pMEA300-derived shuttle vector and characterization of the Amycolatopsis methanolica prephenate dehydratase gene.

By J W Vrijbloed, J van Hylckama Vlieg, N M van der Put, G I Hessels and L Dijkhuizen


An efficient restriction barrier for methylated DNA in the actinomycete Amycolatopsis methanolica could be avoided by using a nonmethylating Escherichia coli strain for DNA isolations. The A. methanolica prephenate dehydratase gene was cloned from a gene bank in a pMEA300-derived shuttle vector in E. coli and characterized

Topics: Research Article
Year: 1995
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Provided by: PubMed Central
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