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The B Subunit of Escherichia coli Heat-Labile Enterotoxin Enhances CD8(+) Cytotoxic-T-Lymphocyte Killing of Epstein-Barr Virus-Infected Cell Lines

By Kong-Wee Ong, A. Douglas Wilson, Timothy R. Hirst and Andrew J. Morgan

Abstract

Epstein-Barr virus (EBV) is associated with a number of important human cancers, including nasopharyngeal carcinoma, gastric carcinoma, and Hodgkin's lymphoma. These tumors express a viral nuclear antigen, EBV nuclear antigen 1 (EBNA1), which cannot be presented to T cells in a major histocompatibility complex class I context, and the viral latent membrane proteins (LMPs). Although the LMPs are expressed in these tumors, no effective immune response is made. We report here that exposure to the cholera-like enterotoxin B subunit (EtxB) in EBV-infected lymphoblastoid cell lines (LCLs) enhances their susceptibility to killing by LMP-specific CD8(+) cytotoxic T lymphocytes (CTLs) in a HLA class I-restricted manner. CTL killing of LCLs is dramatically increased through both transporter-associated protein-dependent and -independent epitopes after EtxB treatment. The use of mutant B subunits revealed that the enhanced susceptibility of LCLs to CTL killing is dependent on the B subunit's interaction with GM(1) but not its signaling properties. These important findings could underpin the development of novel approaches to treating EBV-associated malignancies and may offer a general approach to increasing the presentation of other tumor and viral antigens

Topics: Pathogenesis and Immunity
Publisher: American Society for Microbiology
Year: 2003
DOI identifier: 10.1128/JVI.77.7.4298-4305.2003
OAI identifier: oai:pubmedcentral.nih.gov:150673
Provided by: PubMed Central
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