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Selective Virus Resistance Conferred by Expression of Borna Disease Virus Nucleocapsid Components

By Till Geib, Christian Sauder, Sascha Venturelli, Christel Hässler, Peter Staeheli and Martin Schwemmle

Abstract

Persistent viral infections can render host cells resistant to superinfection with closely related viruses by largely uncharacterized mechanisms. We present evidence for superinfection exclusion in brains of Borna disease virus (BDV)-infected rats and in persistently infected Vero cells, and we suggest that acquired resistance to BDV is due to unbalanced intracellular levels of viral nucleocapsid components. We observed that expression of BDV protein P, N, or X rendered human cells resistant to subsequent challenge with BDV but not with other RNA viruses, indicating that incorrect stoichiometry of nucleocapsid components selectively blocked the polymerase activity of incoming viruses. Vero cells containing high levels of an untranslatable BDV-N transcript remained virus susceptible, demonstrating that viral protein rather than RNA mediated resistance. Transient overexpression of BDV-P in persistently infected Vero cells was also remarkably effective against BDV, indicating that the intracellular balance of viral nucleocapsid components could serve as a target for future therapeutic antiviral strategies

Topics: Virus-Cell Interactions
Publisher: American Society for Microbiology
Year: 2003
DOI identifier: 10.1128/JVI.77.7.4283-4290.2003
OAI identifier: oai:pubmedcentral.nih.gov:150622
Provided by: PubMed Central
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