oaioai:dspace.xmu.edu.cn:2288/170939

Assessing the effects of different processing procedures on the yield of Treponema pallidum DNA from blood

Abstract

背景:聚合酶链反应(Polymerasechainreaction,PCR)可直接检测梅毒螺旋体(Treponemapallidumsubsp.pallidum,T.pallidum),对梅毒进行明确诊断。然而梅毒患者血液样本的T.pallidum-PCR阳性率低,常与临床诊断不符。本研究探讨样本处理流程和血液样本类型对T.pallidumDNA得率的影响,为提高血液T.pallidum-PCR的阳性率提供实验依据。 方法:采用自主构建的Tp47基因荧光定量PCR方法检测一系列体外模拟实验样本和感染兔血中的T.pallidumDNA,探讨不同样本处理流程对T.pallidumDNA提取效率的...Object: Polymerase chain reaction (PCR) is a method to detect the Treponema pallidum subsp. pallidum (T. pallidum) and provides definitive diagnosis of syphilis. However, the sensitivity of T. pallidum blood-PCR is moderate, giving a low level of concordance with the physician’s diagnosis. In this study, we explore a study on the effects of different clinical sample processing procedures and diffe...学位:医学硕士院系专业:医学院_临床检验诊断学学号:2452014115352

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Xiamen University Institutional Repository

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oaioai:dspace.xmu.edu.cn:2288/170939Last time updated on 6/10/2020View original full text link

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