Assessing the effects of different processing procedures on the yield of Treponema pallidum DNA from blood


背景:聚合酶链反应(Polymerasechainreaction,PCR)可直接检测梅毒螺旋体(Treponemapallidumsubsp.pallidum,T.pallidum),对梅毒进行明确诊断。然而梅毒患者血液样本的T.pallidum-PCR阳性率低,常与临床诊断不符。本研究探讨样本处理流程和血液样本类型对T.pallidumDNA得率的影响,为提高血液T.pallidum-PCR的阳性率提供实验依据。 方法:采用自主构建的Tp47基因荧光定量PCR方法检测一系列体外模拟实验样本和感染兔血中的T.pallidumDNA,探讨不同样本处理流程对T.pallidumDNA提取效率的...Object: Polymerase chain reaction (PCR) is a method to detect the Treponema pallidum subsp. pallidum (T. pallidum) and provides definitive diagnosis of syphilis. However, the sensitivity of T. pallidum blood-PCR is moderate, giving a low level of concordance with the physician’s diagnosis. In this study, we explore a study on the effects of different clinical sample processing procedures and diffe...学位:医学硕士院系专业:医学院_临床检验诊断学学号:2452014115352

Similar works

Full text


Xiamen University Institutional Repository

Provided a free PDF
oaioai:dspace.xmu.edu.cn:2288/170939Last time updated on 6/10/2020View original full text link

This paper was published in Xiamen University Institutional Repository.

Having an issue?

Is data on this page outdated, violates copyrights or anything else? Report the problem now and we will take corresponding actions after reviewing your request.