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Mechanism of cis-trans Isomerization of Unsaturated Fatty Acids in Pseudomonas putida

By Angelika von Wallbrunn, Hans Hermann Richnow, Grit Neumann, Friedhelm Meinhardt and Hermann J. Heipieper


We studied the pattern of the cis-trans isomerization of unsaturated fatty acids in cells of Pseudomonas putida S12 grown in a medium supplemented with oleic acid which was deuterated at both of the C atoms of its double bond. Direct evidence that isomerization does not include a transient saturation of the double bond was obtained. In addition, analysis of the amino acid sequences of the seven known Cti proteins identified them as heme-containing proteins of the cytochrome c type

Topics: Enzymes and Proteins
Publisher: American Society for Microbiology
Year: 2003
DOI identifier: 10.1128/JB.185.5.1730-1733.2003
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Provided by: PubMed Central
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