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A new method of synthesis of fluorescently labelled oligonucleotides and their application in DNA sequencing.

By W T Markiewicz, G Gröger, R Rösch, A Zebrowska, M Markiewicz, M Klotz, M Hinz, P Godzina and H Seliger

Abstract

A new approach to the chemical synthesis of oligodeoxynucleotides bearing reporter functional groups at base residues of 3'-end nucleosides is reported. Applications of the 3'-end fluorescently labelled primers for automated DNA sequencing are shown

Topics: Research Article
Year: 1997
DOI identifier: 10.1093/nar/25.18.3672
OAI identifier: oai:pubmedcentral.nih.gov:146946
Provided by: PubMed Central
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