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Cloning of Escherichia coli lacZ and lacY Genes and Their Expression in Gluconobacter oxydans and Acetobacter liquefaciens

By Hesham E. Mostafa, Knut J. Heller and Arnold Geis

Abstract

An efficient transformation protocol for Gluconobacter oxydans and Acetobacter liquefaciens strains was developed by preparation of electrocompetent cells grown on yeast extract-ethanol medium. Plasmid pBBR122 was used as broad-host-range vector to clone the Escherichia coli lacZY genes in G. oxydans and A. liquefaciens. Although both lac genes were functionally expressed in both acetic acid bacteria, only a few transformants were able to grow on lactose. However, this ability strictly depended on the presence of a plasmid expressing both lac genes. Mutations in the plasmids and/or in the chromosome were excluded as the cause of growth ability on lactose

Topics: Physiology and Biotechnology
Publisher: American Society for Microbiology
Year: 2002
DOI identifier: 10.1128/AEM.68.5.2619-2623.2002
OAI identifier: oai:pubmedcentral.nih.gov:127584
Provided by: PubMed Central
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