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Rapid Detection of Shigella Species in Environmental Sewage by an Immunocapture PCR with Universal Primers

By Xuanxian Peng, Wen Luo, Jianying Zhang, Sanying Wang and Shengcai Lin

Abstract

In the present study, we developed a quick, highly specific method for detection of Shigella species by combining immunocapturing of the bacteria and a universal primer PCR. The method drastically enhances test sensitivity, and it can be used not only for identification of Shigella species in the environment but also for rapid detection of other pathogens

Topics: Public Health Microbiology
Publisher: American Society for Microbiology
Year: 2002
DOI identifier: 10.1128/AEM.68.5.2580-2583.2002
OAI identifier: oai:pubmedcentral.nih.gov:127526
Provided by: PubMed Central
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