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Bacilysocin, a Novel Phospholipid Antibiotic Produced by Bacillus subtilis 168

By Norimasa Tamehiro, Yoshiko Okamoto-Hosoya, Susumu Okamoto, Makoto Ubukata, Masa Hamada, Hiroshi Naganawa and Kozo Ochi

Abstract

We have found a novel phospholipid antibiotic (named bacilysocin) which accumulates within (or associates with) the cells of Bacillus subtilis 168 and determined the structure by nuclear magnetic resonance and mass spectrometry analyses. The structure of bacilysocin elucidated was 1-(12-methyltetradecanoyl)-3-phosphoglyceroglycerol. Bacilysocin demonstrated antimicrobial activity, especially against certain fungi. Production of bacilysocin commenced immediately after growth ceased and before the formation of heat-resistant spores. The production of bacilysocin was completely blocked when the ytpA gene, which encodes a protein homologous to lysophospholipase, was disrupted, but blockage of the ytpA gene did not significantly affect growth. Sporulation was also impaired, with a 10-fold reduction in heat-resistant spore titers being detected. Since the ytpA disruptant actually lacked phospholipase activity, we propose that the YtpA protein functions as an enzyme for the biosynthesis of bacilysocin

Topics: Mechanisms of Action: Physiological Effects
Publisher: American Society for Microbiology
Year: 2002
DOI identifier: 10.1128/AAC.46.2.315-320.2002
OAI identifier: oai:pubmedcentral.nih.gov:127064
Provided by: PubMed Central
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