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PCR for Detection of cdt-III and the Relative Frequencies of Cytolethal Distending Toxin Variant-Producing Escherichia coli Isolates from Humans and Cattle

By Clifford G. Clark, Shelley T. Johnson, Russell H. Easy, Jennifer L. Campbell and Frank G. Rodgers

Abstract

A PCR assay that uses primers whose sequences were obtained from the published sequence of the cdt-III gene was developed to determine the frequencies of the cdt-I, cdt-II, and cdt-III genes in Escherichia coli isolates from humans and animals. E. coli isolates producing cytolethal distending toxin (CDT) were infrequently detected. The cdt-I gene was preferentially detected in strains with the cnf1 gene, while the cdt-III gene was found in strains carrying the cnf2 gene. The cdt-III genotype was more prevalent in animal isolates, while the cdt-I and cdt-II genotypes were more evident in human isolates. The presence of further cdt gene variants was indicated by the presence of toxin activity in cell culture in the absence of PCR amplification of the cdt-I, cdt-II, or cdt-III gene

Topics: Bacteriology
Publisher: American Society for Microbiology
Year: 2002
DOI identifier: 10.1128/JCM.40.7.2671-2674.2002
OAI identifier: oai:pubmedcentral.nih.gov:120586
Provided by: PubMed Central
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