Base insertion mutations in the anticodons of two different Escherichia coli tRNAs have been isolated that allow suppression of a series of +1 frameshift mutations. Insertion of a U between positions 34 and 35 of tRNAor addition of a G between positions 36 and 37 of tRNALys expand the anticodons of both tRNAs similarly to 3′−GUUU−5′ and allow decoding of complementary 5′−CAAA−3′ quadruplets. Analysis of the suppressed mRNA sequences suggests that suppression occurs by pairing of the expanded anticodons to all four bases of the complementary, quadruplet codon. The tRNAmutants are identical to the sufG class of frameshift suppressors isolated both in Salmonella enterica serovar Typhimurium and E.coli by Kohno and Roth and previously thought to affect tRNALys
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