Skip to main content
Article thumbnail
Location of Repository

Insertions in the anticodon loop of tRNA1Gln(sufG) and tRNALys promote quadruplet decoding of CAAA

By Michael O’Connor

Abstract

Base insertion mutations in the anticodons of two different Escherichia coli tRNAs have been isolated that allow suppression of a series of +1 frameshift mutations. Insertion of a U between positions 34 and 35 of tRNAor addition of a G between positions 36 and 37 of tRNALys expand the anticodons of both tRNAs similarly to 3′−GUUU−5′ and allow decoding of complementary 5′−CAAA−3′ quadruplets. Analysis of the suppressed mRNA sequences suggests that suppression occurs by pairing of the expanded anticodons to all four bases of the complementary, quadruplet codon. The tRNAmutants are identical to the sufG class of frameshift suppressors isolated both in Salmonella enterica serovar Typhimurium and E.coli by Kohno and Roth and previously thought to affect tRNALys

Topics: Article
Publisher: Oxford University Press
Year: 2002
OAI identifier: oai:pubmedcentral.nih.gov:113831
Provided by: PubMed Central
Sorry, our data provider has not provided any external links therefore we are unable to provide a link to the full text.

Suggested articles


To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.