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Evidence that the Genomic RNA of Hepatitis E Virus Is Capped

By Yamina Kabrane-Lazizi, Xiang-Jin Meng, Robert H. Purcell and Suzanne U. Emerson


Hepatitis E virus (HEV) is an unclassified virus with a positive-sense RNA genome and an undefined replication strategy. In order to determine whether the HEV genome is capped or not, we developed a reverse transcription-PCR assay that is based on the ability of a monoclonal antibody to recognize 7-methylguanosine (m7G). Antibody to m7G bound RNA extracted from virions of two different HEV genotypes. The cap analog competitively inhibited the binding of virion RNAs, demonstrating that HEV has a capped RNA genome

Topics: Structure and Assembly
Publisher: American Society for Microbiology
Year: 1999
OAI identifier: oai:pubmedcentral.nih.gov:112909
Provided by: PubMed Central
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