Location of Repository

Prevotella intermedia Stimulates Expansion of Vβ-Specific CD4+ T Cells

By K.-P. Leung and Barbara A. Torres

Abstract

Recent evidence suggests that certain periodontal pathogens preferentially stimulate T cells expressing specific variable regions on the β chain (Vβ) of the T-cell receptor, which may indicate the presence of a superantigen. Superantigens are microbial proteins that activate large numbers of CD4+ T cells in a Vβ-specific manner. The purpose of this study was to determine whether Prevotella intermedia, a putative periodontal pathogen, activates populations of specific Vβ on CD4+ T cells. Among the bacterial strains tested, P. intermedia strain 17, a clinical isolate, induced the strongest proliferative response in peripheral blood mononuclear cells. Antibodies raised against whole cells of this organism blocked the proliferative activity. P. intermedia-induced proliferation was T-cell specific and required the presence of antigen-presenting cells. Flow cytometric analysis showed that CD4+ T-cell subsets expressing Vβ8, Vβ12, and Vβ17 expanded in response to P. intermedia strain 17. The ability of P. intermedia to stimulate CD4+-T-cell proliferation was further supported by the production profiles of key T-cell cytokines, gamma interferon and interleukin-2. The data collectively suggest that certain strains of P. intermedia can activate Vβ-specific T cells in a manner similar to that of other known microbial superantigens

Topics: Host Response and Inflammation
Publisher: American Society for Microbiology
Year: 2000
OAI identifier: oai:pubmedcentral.nih.gov:101809
Provided by: PubMed Central
Sorry, our data provider has not provided any external links therefor we are unable to provide a PDF.

Suggested articles


To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.