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FspAI, a unique type II restriction endonuclease that recognizes the octanucleotide sequence 5′-RTGC↓GCAY-3′

By A. Kesminiene, Z. Maneliene, J. Vitkute, M. Petruyte and A. Janulaitis

Abstract

A new type II restriction endonuclease designated FspAI has been partially purified from a Flexibacter species Tv-m21K. FspAI recognizes the octanucleotide sequence 5′-RTGC↓GCAY-3′ and cleaves it in the center generating blunt-ended DNA fragments

Topics: NAR Methods Online
Publisher: Oxford University Press
Year: 2001
OAI identifier: oai:pubmedcentral.nih.gov:97628
Provided by: PubMed Central
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