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Reactivity of MEST-1 (Antigalactofuranose) with Trypanosoma cruzi Glycosylinositol Phosphorylceramides (GIPCs): Immunolocalization of GIPCs in Acidic Vesicles of Epimastigotes

By Erika Suzuki, Renato A. Mortara, Helio K. Takahashi and Anita H. Straus

Abstract

Using confocal microscopy, MEST-1-positive immunofluorescence was observed within various Trypanosoma cruzi forms, except in cell-derived trypomastigotes. Glycosylinositol phosphorylceramides were identified by thin-layer chromatography immunostaining as the antigens recognized by MEST-1 in these parasites. In epimastigotes, labeling of MEST-1 coincided with acidic vesicles, indicating an internal localization of these glycoconjugates

Topics: Microbial Immunology
Publisher: American Society for Microbiology
Year: 2001
DOI identifier: 10.1128/CDLI.8.5.1031-1035.2001
OAI identifier: oai:pubmedcentral.nih.gov:96193
Provided by: PubMed Central
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