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SigE Is a Chaperone for the Salmonella enterica Serovar Typhimurium Invasion Protein SigD

By K. Heran Darwin, Lloyd S. Robinson and Virginia L. Miller

Abstract

SigD is translocated into eucaryotic cells by a type III secretion system. In this work, evidence that the putative chaperone SigE directly interacts with SigD is presented. A bacterial two-hybrid system demonstrated that SigE can interact with itself and SigD. In addition, SigD was specifically copurified with SigE-His6 on a nickel column

Topics: Genetics and Molecular Biology
Publisher: American Society for Microbiology
Year: 2001
DOI identifier: 10.1128/JB.183.4.1452-1454.2001
OAI identifier: oai:pubmedcentral.nih.gov:95020
Provided by: PubMed Central
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