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Assembly Requirements and Role of CotH during Spore Coat Formation in Bacillus subtilis

By R. Zilhão, G. Naclerio, A. O. Henriques, L. Baccigalupi, C. P. Moran and E. Ricca


We report Western blot data showing that the 42.8-kDa product of the previously characterized cotH locus (8) is a structural component of the Bacillus subtilis spore coat. We show that the assembly of CotH requires both CotE and GerE. In agreement with these observations, the ultrastructural analysis of purified spores suggests that CotH is needed for proper formation of both inner and outer layers of the coat

Topics: Genetics and Molecular Biology
Publisher: American Society for Microbiology
Year: 1999
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Provided by: PubMed Central
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