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Assembly Requirements and Role of CotH during Spore Coat Formation in Bacillus subtilis

By R. Zilhão, G. Naclerio, A. O. Henriques, L. Baccigalupi, C. P. Moran and E. Ricca

Abstract

We report Western blot data showing that the 42.8-kDa product of the previously characterized cotH locus (8) is a structural component of the Bacillus subtilis spore coat. We show that the assembly of CotH requires both CotE and GerE. In agreement with these observations, the ultrastructural analysis of purified spores suggests that CotH is needed for proper formation of both inner and outer layers of the coat

Topics: Genetics and Molecular Biology
Publisher: American Society for Microbiology
Year: 1999
OAI identifier: oai:pubmedcentral.nih.gov:93693
Provided by: PubMed Central
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