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Isolation of an Active Catalytic Core of Streptococcus downei MFe28 GTF-I Glucosyltransferase

By Vincent Monchois, Martha Arguello-Morales and Roy R. B. Russell

Abstract

Truncated variants of GTF-I from Streptococcus downei MFe28 were purified by means of a histidine tag. Sequential deletions showed that the C-terminal domain was not directly involved in the catalytic process but was required for primer activation. A fully active catalytic core of only 100 kDa was isolated

Topics: Enzymes and Proteins
Publisher: American Society for Microbiology
Year: 1999
OAI identifier: oai:pubmedcentral.nih.gov:93647
Provided by: PubMed Central
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