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A Simple and Reliable Method for Hybridization of Homothallic Wine Strains of Saccharomyces cerevisiae

By Manuel Ramírez, Francisco Peréz and José A. Regodón


A procedure was developed for the hybridization and improvement of homothallic industrial wine yeasts. Killer cycloheximide-sensitive strains were crossed with killer-sensitive cycloheximide-resistant strains to get killer cycloheximide-resistant hybrids, thereby enabling hybrid selection and identification. This procedure also allows backcrossing of spore colonies from the hybrids with parental strains

Topics: Methods
Publisher: American Society for Microbiology
Year: 1998
OAI identifier:
Provided by: PubMed Central
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