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ZnT-3, a putative transporter of zinc into synaptic vesicles

By Richard D. Palmiter, Toby B. Cole, Carol J. Quaife and Seth D. Findley

Abstract

The murine ZnT3 gene was cloned by virtue of its homology to the ZnT2 gene, which encodes a membrane protein that facilitates sequestration of zinc in endosomal vesicles. ZnT-3 protein is predicted to have six transmembrane domains and shares 52% amino acid identity with ZnT-2, with the homology extending throughout the two sequences. Human ZnT-3 cDNAs were also cloned; the amino acid sequence is 86% identical to murine ZnT-3. The mouse ZnT3 gene has 8 exons and maps to chromosome 5. Northern blot and reverse transcriptase–PCR analyses demonstrate that murine ZnT-3 expression is restricted to the brain and testis. In situ hybridization reveals that within the brain, ZnT-3 mRNA is most abundant in the hippocampus and cerebral cortex. Antibodies raised against the C-terminal tail of mouse ZnT-3 react with the projections from these neurons and produce a pattern similar to that obtained with Timm’s reaction, which reveals histochemically reactive zinc within synaptic vesicles. We propose that ZnT-3 facilitates the accumulation of zinc in synaptic vesicles

Topics: Biological Sciences
Publisher: The National Academy of Sciences of the USA
Year: 1996
DOI identifier: 10.1073/pnas.93.25.14934
OAI identifier: oai:pubmedcentral.nih.gov:26240
Provided by: PubMed Central
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