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Persistent expression of human clotting factor IX from mouse liver after intravenous injection of adeno-associated virus vectors

By Dwight D. Koeberl, Ian E. Alexander, Christine L. Halbert, David W. Russell and A. Dusty Miller

Abstract

We previously found that gene transduction by adeno-associated virus (AAV) vectors in cell culture can be stimulated over 100-fold by treatment of the target cells with agents that affect DNA metabolism, such as irradiation or topoisomerase inhibitors. Here we show that previous γ-irradiation increased the transduction rate in mouse liver by up to 900-fold, and the topoisomerase inhibitor etoposide increased transduction by about 20-fold. Similar rates of hepatic transduction were obtained by direct injection of the liver or by systemic delivery via tail vein injection. Hepatocytes were much more efficiently transduced than other cells after systemic delivery, and up to 3% of all hepatocytes could be transduced after one vector injection. The presence of wild-type AAV, which contaminates many AAV vector preparations, was required to observe a full response to γ-irradiation. Injection of mice with AAV vectors encoding human clotting factor IX after γ-irradiation resulted in synthesis of low levels of human clotting factor IX for the 5-month period of observation. These studies show the potential of targeted gene transduction of the liver by AAV vectors for treatment of various hematological or metabolic diseases

Topics: Biological Sciences
Publisher: The National Academy of Sciences of the USA
Year: 1997
DOI identifier: 10.1073/pnas.94.4.1426
OAI identifier: oai:pubmedcentral.nih.gov:19807
Provided by: PubMed Central
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