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Characterization of individual polynucleotide molecules using a membrane channel

By John J. Kasianowicz, Eric Brandin, Daniel Branton and David W. Deamer


We show that an electric field can drive single-stranded RNA and DNA molecules through a 2.6-nm diameter ion channel in a lipid bilayer membrane. Because the channel diameter can accommodate only a single strand of RNA or DNA, each polymer traverses the membrane as an extended chain that partially blocks the channel. The passage of each molecule is detected as a transient decrease of ionic current whose duration is proportional to polymer length. Channel blockades can therefore be used to measure polynucleotide length. With further improvements, the method could in principle provide direct, high-speed detection of the sequence of bases in single molecules of DNA or RNA

Topics: Biological Sciences
Publisher: The National Academy of Sciences of the USA
Year: 1996
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Provided by: PubMed Central
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