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CBP/p300 interact with and function as transcriptional coactivators of BRCA1

By Gerald M. Pao, Ralf Janknecht, Heinz Ruffner, Tony Hunter and Inder M. Verma

Abstract

BRCA1 is a breast and ovarian cancer-specific tumor suppressor, with properties of a transcription factor involved in DNA repair. We previously have shown the transactivation of heterologous promoters by the carboxyl terminus of BRCA1. We now describe that BRCA1-mediated transactivation is enhanced by p300/CBP (CREB binding protein) and that this effect was suppressed by the adenovirus E1A oncoprotein. We show a physical association of BRCA1 with the transcriptional coactivators/acetyltransferases p300 and CBP. Endogenous as well as overexpressed BRCA1 and p300 were found to associate in a phosphorylation-independent manner. BRCA1 interacts with the cAMP response element binding protein (CREB) domain of p300/CBP via both its amino and carboxyl termini. Finally, full-length BRCA1 is shown to transcriptionally activate the Rous sarcoma virus-long terminal repeat promoter, which was further stimulated by p300. Immunocolocalization analyses suggest that BRCA1 and p300 associate in a cell cycle-dependent manner. Our results support a role for BRCA1 in transcription

Topics: Biological Sciences
Publisher: The National Academy of Sciences
Year: 2000
DOI identifier: 10.1073/pnas.97.3.1020
OAI identifier: oai:pubmedcentral.nih.gov:15508
Provided by: PubMed Central
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