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DETECTION OF A t -COMPLEX ANTIGEN BY SECONDARY CELL-MEDIATED LYMPHOCYTOTOXICITY

By C. Hammerberg

Abstract

Because of the inconsistency in published results concerning the serological detection of cell surface antigens coded for by the t -complex, a cell-mediated lymphocytotoxicity (CML) assay, secondary CML, was used in a search for t -antigens. By sensitizing C3H. Ttf (C3H. Brachyury, tufted) with the congenic strain C3H. Ttf/t w18 splenic cells, a response against lipopolysaccharide (LPS) stimulated splenic cells from C3H. Ttf/t w18 mice is obtained. The locus coding for the antigen detected by this reaction lies to the left of tf on the murine seventeenth chromosome. The secondary CML response to this antigen is H-2 restricted and detects an antigen on all t -haplotypes tested: t w18 , t w18 tf, t 12 , t 6 , t h2 tf, and t w5

Publisher: Blackwell Publishing Ltd
Year: 1982
DOI identifier: 10.1111/j.1744-313X.1982.tb00789.x
OAI identifier: oai:deepblue.lib.umich.edu:2027.42/75626
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