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Inhibition of Interleukin-1-Induced Effects in Synoviocytes Transduced with the Human IL-1 Receptor Antagonist cDNA Using an Adenoviral Vector

By Blake J. Roessler, John W. Hartman, David K. Vallance, Jill M. Latta, Sonia L. Janich and Beverly L. Davidson

Abstract

Overview summary Adenovirus-mediated gene transfer into the cells of the synovial membrane may provide a means to deliver therapeutically active proteins for the local modification of the immune response in inflammatory arthropathies. In this study, we infected type B human synoviocytes in vitro and rabbit synovial lining membrane in vivo with a recombinant human adenovirus containing the cDNA for the human interleukin-1 receptor antagonist protein (IL-1ra). Expression of human IL-1ra was observed both in the transduced synoviocytes in vitro and in the microenvironment of the transduced rabbit synovial membrane in vivo, and the functional activity of the transgenic IL-1ra was suggested by in vitro inhibition of interleukin-1 (IL-1)-induced prostaglandin E2 (PGE2) production and by in vivo inhibition of IL-1-induced glycosaminoglycan (GAG) degradation

Publisher: Mary Ann Liebert, Inc., publishers
Year: 1995
DOI identifier: 10.1089/hum.1995.6.3-307
OAI identifier: oai:deepblue.lib.umich.edu:2027.42/63124
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