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Effect of fixation temperature on flow cytometric measurement of intracellular antibody content of hybridomas during batch culture

By Gyun Min Lee, Alice S. Chuck and Bernhard Ø Palsson

Abstract

In order to investigate the effect of fixation temperature on flow cytometric measurement of intracellular antibody content of hybridoma cells, cells in different growth stages during a batch culture were fixed and stored at 4 and -20 °C, respectively. Flow cytometric analysis indicates that both fixation temperatures can be used in monitoring the changes in intracellular antibody content of the cells during a batch culture. However, it is better to fix and store the cells at -20 °C than 4 °C with regard to preservation of intracellular antibody and storage stability

Publisher: Kluwer Academic Publishers; Science & Technology Letters ; Springer Science+Business Media
Year: 1993
DOI identifier: 10.1007/BF00150897
OAI identifier: oai:deepblue.lib.umich.edu:2027.42/42491
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