Skip to main content
Article thumbnail
Location of Repository

The NH2-Terminal Region of Rabbit CYP2E1 Is Not Essential for Interaction with NADPH-Cytochrome P450 Reductase

By Voznesensky A. I., Schenkman J. B., Pernecky S. J. and Coon M. J.

Abstract

Recently we reported that electrostatic forces interfere with the formation of the NADPH-cytochrome P450 reductase/cytochrome P450 electron transfer complex and suggested that this complex is formed by the attraction of the complementary hydrophobic patches (Voznesensky, A., and Schenkman,J.(1994) 269 J.Biol.Chem, 15724-1573). In this report we evaluate the role of the NH2-terminal hydrophobic region of CYP2E1 in the interaction with the reductase by comparing ionic strength dependence of the reduction of the full-length and truncated [Delta]3-29 CYP2E1. Increasing ionic strength stimulates reduction of both full-length and truncated CYP2E1. The neutralization of electrostatic interactions by increasing ionic strength revealed no impairment of the [Delta]3-29 CYP2E1 reduction compared to that of the full-length CYP2E1, indicating that the NH2-terminal region is not essential for the interaction of the cytochrome with the reductase

Publisher: Elsevier
Year: 1994
DOI identifier: 10.1006/bbrc.1994.2162
OAI identifier: oai:deepblue.lib.umich.edu:2027.42/31380
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • http://www.sciencedirect.com/s... (external link)
  • http://hdl.handle.net/2027.42/... (external link)
  • http://www.ncbi.nlm.nih.gov/si... (external link)
  • http://dx.doi.org/10.1006/bbrc... (external link)
  • Suggested articles


    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.