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Bionanoconjugates of tyrosinase and peptide-derivatised gold nanoparticles for biosensing of phenolic compounds

By J Cortez, E Vorobieva, D Gralheira, I Osorio, L Soares, N Vale, E Pereira, P Gomes and R Franco

Abstract

Bionanoconjugates of the enzyme tyrosinase (TYR) and gold nanoparticles (AuNPs) functionalised with a peptide (CALNN) were produced in solution and characterised. The formation of stable TYR-AuNP:CALNN bionanoconjugates (BNCs) was supported by a decrease of the surface charge of the BNCs as determined by zeta-potential and an increase in hydrodynamic diameter as determined by Dynamic Light Scattering (DLS). UV/Vis studies of pH-induced aggregation revealed distinct protonation patterns for the BNCs when compared with AuNP:CALNN alone, further substantiating BNC formation. Activity studies of the BNCs for the reduction of di-phenols in solution indicated that TYR not only remains active after conjugation, but interestingly its activity in the BNCs is higher than for the free enzyme. In conclusion, AuNP:CALNN can provide a suitable platform for the immobilisation of TYR, leading to BNCs with increased enzyme activity and a wider pH working range, with promising uses in electrochemical biosensors for the detection of mono- and di-phenolic compounds

Topics: Engenharia dos materiais, Materials engineering, Ciências da engenharia e tecnologias::Engenharia dos materiais, Engineering and technology::Materials engineering
Publisher: 'Springer Science and Business Media LLC'
Year: 2011
DOI identifier: 10.1007/s11051-010-0099-8
OAI identifier: oai:repositorio-aberto.up.pt:10216/82098
Journal:

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