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Production of siderophore type chelates by mixed bacterioplankton populations in nutrient enriched seawater incubations

By M. Gledhill, P. McCormack, S. Ussher, E.P. Achterberg, R.F.C. Mantoura and P. Worsfold

Abstract

Siderophore type chelates were isolated from nutrient enriched seawater collected from coastal and near shore environments and detected using a novel high performance liquid chromatography – electrospray ionisation – mass spectrometric technique. Seawater was enriched with added glucose, ammonia and phosphate, and incubated for four days. Seven different siderophore type compounds were detected in the extracted supernatants and tentatively identified based on mass numbers and spectra. The compounds comprised two groups, the ferrioxamines and the amphibactins. They were produced at typical coastal iron concentrations (total dissolved iron = 2.9 +/- 1.4 nM and 2.2 +/- 0.1 nM) both in the presence and absence of the iron chelating ligand ethylene diamine-N,N-diacetic acid

Topics: QD, QR, GC
Year: 2004
OAI identifier: oai:eprints.soton.ac.uk:37736
Provided by: e-Prints Soton

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  1. (1993). Aerobactin production by a planktonic marine Vibrio sp.
  2. (1998). Biogeochemical controls and feedbacks on ocean primary productivity.
  3. (2002). Biogeochemistry of Fe and other trace elements (Al, Co, Ni) in the upper Atlantic Ocean. Deep-Sea Res.
  4. (2000). Carbon versus iron limitation of bacterial growth in the Californian upwelling regime.
  5. (2001). Collection and detection of natural iron-binding ligands from seawater.
  6. (1999). Competition among marine phytoplankton for different chelated iron species.
  7. (1995). Complexation of iron(III) by natural organic ligands as determined by a new competitive equilibration/adsorptive cathodic stripping voltammetry method.
  8. (1998). Critically selected stability constants of metal complexes version 5.0.
  9. (1994). Deferration of laboratory media and assays for ferric and ferrous ions.
  10. (2000). Determination of conditional stability constants and kinetic constants for strong model Fe-binding ligands in seawater.
  11. (2001). Determination of iron in seawater.
  12. (1993). Direct estimates of bacterial numbers in seawater samples without incurring cell loss due to sample
  13. (2000). Electrospray Ionisation – Mass Spectrometry of Hydroxamate siderophores.
  14. (1997). Evidence for organic complexation of iron in seawater.
  15. (1989). Hydroxamate -siderophore production and utilization by marine eubacteria.
  16. (2001). Identification of a natural desferrioxamine siderophore produced by a marine bacterium.
  17. (1996). Iron stimulation of antarctic bacteria.
  18. (2002). Membrane affinity of the amphiphilic marinobactin siderophores.
  19. (1988). Northeast Pacific iron distributions in relation to phytoplankton productivity.
  20. (2001). Photochemical cycling of iron in the surface ocean mediated by microbial iron(III)-binding ligands.
  21. (2003). Photochemical reactivity of siderophores produced by marine heterotrophic bacteria and cyanobacteria based on characteristic Fe(III) binding groups.
  22. (1988). Preparation and chemistry of the artificial algal culture medium Aquil.
  23. (1983). Prorocentrin: an extracellular siderophore produced by the marine dinoflagellate Prorocentrum minimum.
  24. (2003). Separation and detection of siderophores produced by marine bacterioplankton using highperformance liquid chromatography with electrospray ionization mass spectrometry.
  25. (2001). Siderophores in marine coastal waters and their relevance for iron uptake by phytoplankton: experiments with the diatom Phaeodactylum tricornutum.
  26. (1995). Siderophores: structure and function of microbial iron transport compounds.
  27. (2003). Structure and membrane affinity of a suite of amphiphilic siderophores produced by a marine bacterium.
  28. (1991). Structures, coordination chemistry and functions of microbial iron chelates.
  29. (1996). The role of heterotrophic bacteria in iron-limited ocean ecosystems.
  30. (1999). Utilization of iron bound to strong organic ligands by plankton communities in the subarctic Pacific Ocean.
  31. (1998). Variability in the speciation of iron in the northern North Sea.
  32. (1995). Voltammetric estimation of iron (III) thermodynamic 17 stability constants for chatecholate siderophores isolated from marine bacteria and cyanobacteria.

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