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Growth kinetics of Mycobacterium tuberculosis measured by quantitative resazurin reduction assay: a tool for fitness studies

By A. Von Groll, A. Martin, F. Portaels, P. E. Almeida da Silva and J. C. Palomino

Abstract

We standardized a method to evaluate the growth kinetics of Mycobacterium tuberculosis by measuring quantitatively the reduction of resazurin by spectrophotometry. Growth curves and the rate of growth of twenty-one M. tuberculosis clinical isolates were determined. The method showed technical simplicity and is inexpensive to assess the fitness of each isolate

Topics: Bacterial diseases, Tuberculosis, Mycobacterium tuberculosis, Growth, Kinetics, Measurement, Resazurin, Assays, Laboratory techniques and procedures, Fitness inheritance, Quantification
Year: 2010
DOI identifier: 10.1590/S1517-83822010000200006
OAI identifier: oai:dspace.itg.be:10390/6128
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