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SNP markers for early identification of high molecular weight glutenin subunits (HMW-GSs) in bread wheat

By Catherine Ravel, Annie Faye, Sarah Ben-Sadoun, Marion Ranoux, Mireille Dardevet, Cécile Dupuits, Florence Exbrayat, Charles Poncet, Pierre Sourdille and Gerard Branlard

Abstract

Key message A set of eight SNP markers was developed to facilitate the early selection of HMW-GS alleles in breeding programmes. In bread wheat (Triticum aestivum), the high molecular weight glutenin subunits (HMW-GSs) are the most important determinants of technological quality. Known to be very diverse, HMW-GSs are encoded by the tightly linked genes Glu-1-1 and Glu-1-2. Alleles that improve the quality of dough have been identified. Up to now, sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) of grain proteins is the most widely used for their identification. To facilitate the early selection of HMW-GS alleles in breeding programmes, we developed DNA-based molecular markers. For each accession of a core collection (n = 364 lines) representative of worldwide bread wheat diversity, HMW-GSs were characterized by both genotyping and SDS-PAGE. Based on electrophoresis, we observed at least 8, 22 and 9 different alleles at the Glu-A1, Glu-B1 and Glu-D1 loci, respectively, including new variants. We designed a set of 17 single-nucleotide polymorphism (SNP) markers that were representative of the most frequent SDS-PAGE alleles at each locus. At Glu-A1 and Glu-D1, two and three marker-based haplotypes, respectively, captured the diversity of the SDS-PAGE alleles rather well. Discrepancies were found mainly for the Glu-B1 locus. However, statistical tests revealed that two markers at each Glu-B1 gene and their corresponding haplotypes were more significantly associated with the rheological properties of the dough than were the relevant SDS-PAGE alleles. To conclude, this study demonstrates that the SNP markers developed provide additional information on HMW-GS diversity. Two markers at Glu-A1, four at Glu-B1 and two at Glu-D1 constitute a useful toolbox for breeding wheat to improve end-use value

Topics: Glutenin, Molecular marker, Allele, Bread wheat, Allèle, Blé panifiable, Gluténine, Marqueur moléculaire, MESH: Triticum aestivum, MESH: Polymorphisme de nucléotide simple, [SDV.SA.STA]Life Sciences [q-bio]/Agricultural sciences/Sciences and technics of agriculture, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.GEN.GPL]Life Sciences [q-bio]/Genetics/Plants genetics
Publisher: 'Springer Science and Business Media LLC'
Year: 2020
DOI identifier: 10.1007/s00122-019-03505-y
OAI identifier: oai:HAL:hal-02528930v1
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