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KARAKTERISASI KROMOSOM SERANGGA PENGHASIL SUTERA LIAR Attacus atlas ( L) (LEPIDOPTERA : SATURNIIDAE)

By , ERNAWATI, SPd. and , Dr. Niken Satuti N.H., M.Sc.

Abstract

A. atlas silk moth produces silk fiber high economic value. Research on wild silk moths has been done in Indonesia, including, rearing in space and in the field, artificial feeding and the kind of silk fiber produced. Genetic studies for wild silkmoth A. atlas have not done a lot. These cytogenetic studies as preliminary research that aims to know the characters, especially in terms of chromosome number, shape and size of A. atlas chromosomes. Sampling was conducted in Kalitirto village, Berbah Subdistric, Sleman Regency, Yogyakarta Province. The samples used were last instar ofA. atlas. The study was conducted at the Entomology Laboratory of Biology Faculty Universitas Gadjah Mada in January to July 2011. Chromosome preparation was performed using smear methods and materials preparation using last instar ovary, at the pachitene stage of meiotic I division cell occurs. Procedure preparation phase of meiotic chromosomes in the study followed the procedures Gupta and Narang (1981). Chromosome analysis performed after staining with Giemsa. The results showed that A. atlas chromosome are numerous, ie haploid chromosomes n = 31,32,33,34, chromosoms shape holocentric type (have a lot of the centromere) and small size +,9773 to 0,7123µm. Variations in chromosome number proceed from holocentric chromosoms are easy due to the fragmented and there are supernurerary chromosomes

Topics: ETD
Publisher: [Yogyakarta] : Universitas Gadjah Mada
Year: 2011
OAI identifier: oai:generic.eprints.org:90492/core8303
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