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Novel method for detection, typing, and quantification of human papillomaviruses in clinical samples

By Keith William Hart, O. Martin Williams, Nicola Thelwell, Alison Nina Fiander, Tom Brown, Lesek K. Borysiewicz and Colin M. Gelder

Abstract

We report the development of a novel detection and typing methodology for human papillomaviruses (HPV) based on real-time PCR with the self-probing fluorescent primers known as Scorpions. This technique is quick, simple, specific, sensitive, and capable of estimating viral load per cell. We report the results of over 100 typing reactions performed on cell lines, biopsies, and cervical cytobrush samples which, when compared to the current reference HPV detection and typing technique, present a kappa value of 0.89. We further report preliminary data suggesting a relationship between viral load per cell and grade of cervical disease

Topics: R Medicine (General), RZ Other systems of medicine
Publisher: American Society for Microbiology
Year: 2001
DOI identifier: 10.1128/jcm.39.9.3204-3212.2001
OAI identifier: oai:http://orca.cf.ac.uk:67632
Provided by: ORCA
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