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Investigation of the Efficiencies of Bioaerosol Samplers for Collecting Aerosolized Bacteria Using a Fluorescent Tracer. I: Effects of Non-sampling Processes on Bacterial Culturability

By Y. Zhao, A.J.A. Aarnink, P. Doornenbal, T.T.T. Huynh, P.W.G. Groot Koerkamp, de M.C.M. Jong and W.J.M. Landman

Abstract

By sampling aerosolized microorganisms, the efficiency of a bioaerosol sampler can be calculated depending on its ability both to collect microorganisms and to preserve their culturability during a sampling process. However, those culturability losses in the non-sampling processes should not be counted toward the sampling efficiency. Prior to the efficiency assessment, this study was designed to investigate the culturability losses in three non-sampling processes: (1) the tracer uranine induced loss; (2) the loss during aerosolization (pre-sampling process); and (3) the bacteria and uranine recovery in air sample handling procedures for the samples of the Andersen 6-stage impactor and the Airport MD8 (post-sampling process). The results indicated that uranine had no significant effect on the culturability of Enterococcus faecalis, Escherichia coli, and Mycoplasma synoviae in suspensions (P > 0.05), but negatively affected the culturability of Campylobacter jejuni (P = 0.01). The culturability of E. faecalis, E. coli, and M. synoviae was not affected by stresses caused by aerosolization (P > 0.05). Only 29% of C. jejuni were still culturable during aerosolization (P = 0.02). In the air sample handling procedures, the four species of bacteria were recovered without significant losses from the samples of the Andersen impactor, but only 33-60% uranine was recovered. E. faecalis, E. coli, and M. synoviae were recovered without significant losses from the samples of the Airport MD8. More C. jejuni was recovered (172%), probably due to multiplication or counting variation. It is suggested that tracer and bacteria should be aerosolized separately when the tracer negatively affects the bacterial culturability. In both pre- and post-sampling processes, losses of bacterial culturability (or multiplication) may occur, which should be taken into account when assessing the efficiencies of bioaerosol samplers

Topics: airborne particles, relative-humidity, survival, rotavirus, swine, air, microorganisms, temperature, enumeration, barns
Year: 2011
DOI identifier: 10.1080/02786826.2010.543196
OAI identifier: oai:library.wur.nl:wurpubs/406118
Provided by: Wageningen Yield
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