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Registration of T-2 mycotoxin with total internal reflection ellipsometry and QCM impedance methods

By A. V. Nabok, A. Tsargorodskaya, A. Holloway, N. F. Starodub and O. Gojster


A sensitive optical method of total internal reflection ellipsometry (TIRE) in conjunction with immune assay approach was exploited for the registration of T-2 mycotoxin in a wide range of concentrations from 100 mu g/mI down to 0.15 ng/ml. Association constants of 1.4 x 10(6) and 1.9 x 10(7) mol(-1) s for poly- and monoclonal T-2 antibodies, respectively, were evaluated from TIRE kinetic measurements. According to TIRE data fitting, binding of T-2 molecules to antibodies (at saturation) has resulted in the increase in adsorbed layer thickness of 4-5 nm. The QCM impedance measurements data showed anomalously large mass increase and film softening, most likely, due to the binding of large T-2 aggregates to antibodies. (c) 2006 Elsevier B.V. All rights reserved

Year: 2007
DOI identifier: 10.1016/j.bios.2006.03.010
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