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Depth profiling in membranes by fluorescence quenching

By Amitabha Chattopadhyay

Abstract

Membrane penetration depth is an important parameter in relation to membrane structure and organization. A methodology has been developed to analyze the membrane penetration depths of fluorescent molecules or groups utilizing differential fluorescence quenching caused by membrane embedded spin-label probes located at different depths. The method involves determination of the parallax in the apparent location of fluorophores, detected when quenching by phospholipids spin-labelled at two different depths is compared. By use of relatively simple algebraic expressions, the method allows calculation of depth in λ . This method has been used to determine the location of fluorophores in NBD-labelled lipids and anthroyloxy-labelled fatty acids in model membranes and of the membrane embedded tryptophan residues in the reconstituted nicotinic acetylcholine receptor

Topics: QH301 Biology
Publisher: 'Springer Science and Business Media LLC'
Year: 1990
DOI identifier: 10.1007/BF02703874
OAI identifier: oai:repository.ias.ac.in:6985

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