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The ArathEULS3 lectin ends up in stress granules and can follow an unconventional route for secretion

By Malgorzata Dubiel, Tibo De Coninck, Vinicius Da Silva Osterne, Isabel Verbeke, Daniël Van Damme, Guy Smagghe and Els Van Damme

Abstract

Stress granules are cytoplasmic compartments, which serve as mRNA storage units during stress, therefore regulating translation. The Arabidopsis thaliana lectin ArathEULS3 has been widely described as a stress inducible gene. This study aimed to examine in detail the localization of ArathEULS3 lectin in normal and stressed cells. Colocalization experiments revealed that the nucleo-cytoplasmic lectin ArathEULS3 relocates to stress granules after stress. The ArathEULS3 sequence encodes a protein with a EUL lectin domain and an N-terminal domain with unknown structure and function. Bioinformatics analyses showed that the N-terminal domain sequence contains intrinsically disordered regions and likely does not exhibit a stable protein fold. Plasmolysis experiments indicated that ArathEULS3 also localizes to the apoplast, suggesting that this protein might follow an unconventional route for secretion. As part of our efforts we also investigated the interactome of ArathEULS3 and identified several putative interaction partners important for the protein translation process

Topics: Biology and Life Sciences, Physical and Theoretical Chemistry, Inorganic Chemistry, Organic Chemistry, Spectroscopy, Molecular Biology, Catalysis, General Medicine, Computer Science Applications, Arabidopsis, ArathEULS3, intrinsically disordered regions, plant lectin, stress granules, unconventional protein secretion, INTRINSICALLY DISORDERED REGIONS, EXTRACELLULAR VESICLES, PROCESSING BODIES, PLANT-LECTINS, ARABIDOPSIS, PROTEIN, COMPONENTS, PREDICTION, DISTINCT, GROWTH
Publisher: 'MDPI AG'
Year: 2020
DOI identifier: 10.3390/ijms21051659
OAI identifier: oai:archive.ugent.be:8652914

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